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superscript iv vilo master mix 11756050  (Thermo Fisher)


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    Thermo Fisher superscript iv vilo master mix 11756050
    Superscript Iv Vilo Master Mix 11756050, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iv+vilo+master+mix+11756050/high+capacity+cdna+reverse+transcription+kit/pmc12255046-35-18-24
    Average 90 stars, based on 1 article reviews
    superscript iv vilo master mix 11756050 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: A cohesin traffic pattern genetically linked to gene regulation
    Article Snippet: Reverse transcription was performed with VILO IV (SuperScript TM IV VILO TM Master Mix, 11756050, Thermo Fisher Scientific) and incubated 20 minutes at 25°C, 10 minutes at 50°C and 5 minutes at 85°C. cDNA was diluted by 20 and 2μL was used for the qPCR. qPCR qPCR was directly done on the cDNAs using Fast SYBR TM Green Master Mix (ThermoFisher Scientific, 4385612) and analyzed on a StepOnePlus TM Real-Time PCR System (ThermoFisher Scientific) using StepOne TM Plus v2.3 software.

    Polymerase Chain Reaction:

    Article Title: Survey of white-footed mice ( Peromyscus leucopus ) in Connecticut, USA reveals low SARS-CoV-2 seroprevalence and infection with divergent betacoronaviruses
    Article Snippet: .. For the pan-coronavirus PCR testing, we first synthesized cDNA from the nucleic acid extracted during SARS-CoV-2 RT-qPCR testing using 4 μl of SuperScript IV VILO Master Mix (ThermoFisher Scientific 11756050), 6 μl of nuclease-free water, and 10 μl of nucleic acid per reaction (Fig. and Supplementary Fig. ). ..

    Synthesized:

    Article Title: Survey of white-footed mice ( Peromyscus leucopus ) in Connecticut, USA reveals low SARS-CoV-2 seroprevalence and infection with divergent betacoronaviruses
    Article Snippet: .. For the pan-coronavirus PCR testing, we first synthesized cDNA from the nucleic acid extracted during SARS-CoV-2 RT-qPCR testing using 4 μl of SuperScript IV VILO Master Mix (ThermoFisher Scientific 11756050), 6 μl of nuclease-free water, and 10 μl of nucleic acid per reaction (Fig. and Supplementary Fig. ). ..

    Article Title: Unveiling the potential of eDNA/eRNA approaches for monitoring the critically endangered Yangtze finless porpoise
    Article Snippet: .. The eRNA was eluted in 100 μL RNase-free water and 60 μL was immediately used for cDNA synthesis, while the remaining 40 μL was archived at − 80◦C. cDNA was synthesized from 60 μL eRNA using SuperScript IV VILO Master Mix (Thermo Fisher Scientific, Cat. 11756050). ..

    Article Title: R-spondins secreted by human pancreas-derived mesenchymal stromal cells support pancreatic organoid proliferation.
    Article Snippet: Total RNA was quantified, and quality checked by NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. For the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay, cDNA was synthesized with SuperScript IV VILO Master Mix (11,756,050; Thermo Fisher Scientific). .. The resulting cDNA was diluted 1:10 in water and 1 μL was used as a template for qRT-PCR analysis with PowerUp SYBR Green Master Mix (A15780; Applied Biosystems) in a CFX96 thermal cycler (BioRad).

    Quantitative RT-PCR:

    Article Title: Survey of white-footed mice ( Peromyscus leucopus ) in Connecticut, USA reveals low SARS-CoV-2 seroprevalence and infection with divergent betacoronaviruses
    Article Snippet: .. For the pan-coronavirus PCR testing, we first synthesized cDNA from the nucleic acid extracted during SARS-CoV-2 RT-qPCR testing using 4 μl of SuperScript IV VILO Master Mix (ThermoFisher Scientific 11756050), 6 μl of nuclease-free water, and 10 μl of nucleic acid per reaction (Fig. and Supplementary Fig. ). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The SARS-CoV-2 envelope PDZ binding motif acts as a virulence factor disrupting host’s epithelial cell–cell junctions
    Article Snippet: .. Viral fragments were obtained either by RT-PCR on RNA extracted from Vero-E6 cells infected by SARS-CoV-2 using the SuperScript IV VILO Master Mix (11756050, Thermo Fisher Scientific ) according to the manufacturer’s protocol, or using synthetic genes ( GeneArt ). .. All PCR amplifications were performed using Phusion TM High-Fidelity DNA Polymerase (F530, Thermofisher ).

    Article Title: R-spondins secreted by human pancreas-derived mesenchymal stromal cells support pancreatic organoid proliferation.
    Article Snippet: Total RNA was quantified, and quality checked by NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. For the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay, cDNA was synthesized with SuperScript IV VILO Master Mix (11,756,050; Thermo Fisher Scientific). .. The resulting cDNA was diluted 1:10 in water and 1 μL was used as a template for qRT-PCR analysis with PowerUp SYBR Green Master Mix (A15780; Applied Biosystems) in a CFX96 thermal cycler (BioRad).

    Infection:

    Article Title: The SARS-CoV-2 envelope PDZ binding motif acts as a virulence factor disrupting host’s epithelial cell–cell junctions
    Article Snippet: .. Viral fragments were obtained either by RT-PCR on RNA extracted from Vero-E6 cells infected by SARS-CoV-2 using the SuperScript IV VILO Master Mix (11756050, Thermo Fisher Scientific ) according to the manufacturer’s protocol, or using synthetic genes ( GeneArt ). .. All PCR amplifications were performed using Phusion TM High-Fidelity DNA Polymerase (F530, Thermofisher ).

    Article Title: Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants.
    Article Snippet: .. Viral fragments were obtained either by RT-PCRonRNA extracted from Vero-E6 cells infected by Delta using the SuperScript IV VILO Master Mix (11756050, Thermofisher) according to the manufacturer protocol, or using synthetic genes (GeneArt, Life Technologies). .. All PCR amplifications were performed using PhusionTMHigh-Fidelity DNA Polymerase (F530, Thermofisher).

    cDNA Synthesis:

    Article Title: Unveiling the potential of eDNA/eRNA approaches for monitoring the critically endangered Yangtze finless porpoise
    Article Snippet: .. The eRNA was eluted in 100 μL RNase-free water and 60 μL was immediately used for cDNA synthesis, while the remaining 40 μL was archived at − 80◦C. cDNA was synthesized from 60 μL eRNA using SuperScript IV VILO Master Mix (Thermo Fisher Scientific, Cat. 11756050). ..

    Reverse Transcription:

    Article Title: NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages
    Article Snippet: .. To determine levels of NLRP11 , CASP4 , and GSDMD transcripts, either at baseline or following siRNA, reverse transcription followed by real-time quantitative PCR (RT-qPCR) was performed using SuperScript IV VILO Master Mix (ThermoFisher Scientific, 11756050), SsoFast EvaGreen Supermix (BioRad, #172–5201), and the QuantStudio TM 5 Real-Time PCR System, 384-well, real-time PCR detection system (Applied Biosystems, A28140) with cycles of 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 20–30 s at 60°C. .. Relative expression levels were calculated using the manufacturer’s software for double delta threshold cycle (Ct) method (Design and Analysis Software 2.6.0, Applied Biosystems), using transcripts from housekeeping genes ACTB and GAPDH (for CASP4 and GSDMD ) and HPRT1 (for NLRP11 ).

    Real-time Polymerase Chain Reaction:

    Article Title: NLRP11 is a pattern recognition receptor for bacterial lipopolysaccharide in the cytosol of human macrophages
    Article Snippet: .. To determine levels of NLRP11 , CASP4 , and GSDMD transcripts, either at baseline or following siRNA, reverse transcription followed by real-time quantitative PCR (RT-qPCR) was performed using SuperScript IV VILO Master Mix (ThermoFisher Scientific, 11756050), SsoFast EvaGreen Supermix (BioRad, #172–5201), and the QuantStudio TM 5 Real-Time PCR System, 384-well, real-time PCR detection system (Applied Biosystems, A28140) with cycles of 30 s at 95°C, followed by 40 cycles of 5 s at 95°C and 20–30 s at 60°C. .. Relative expression levels were calculated using the manufacturer’s software for double delta threshold cycle (Ct) method (Design and Analysis Software 2.6.0, Applied Biosystems), using transcripts from housekeeping genes ACTB and GAPDH (for CASP4 and GSDMD ) and HPRT1 (for NLRP11 ).



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